Simulation of Human Luteal Endocrine Function with Granulosa Lutein Cell Culture*
نویسندگان
چکیده
منابع مشابه
Comparison of nine media in the culture of human ovarian granulosa lutein cells.
BACKGROUND Cultures of human ovarian granulosa lutein (hGL) cells are broadly used in experimental studies. The choice of the culture medium is important for the optimization of the conditions for culture of hGL cells. AIM To compare the efficiency of a basic salt solution and eight different defined media on the culture of hGL cells. MATERIALS AND METHODS Cultures of the HGL-5 cell line we...
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Corpora lutea from Sprague-Dawley rats that were orally administered 0.0 (control), 1.0, 10.0, and 100.0 mglkg hexachlorobenzene (HCB) for 21 days were analyzed by electron microscopy. Granulosa lutein cells (GLC) from animals of the 10.0 mg group showed differences from the cells of animals that served as the controls. Golgi complexes and smooth endoplasmic reticulum appeared more conspicuous,...
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Link protein (LP), an extracellular matrix protein in cartilage, stabilizes aggregates of hyaluronic acid (HA) and proteoglycans, including aggrecan and inter-alpha-trypsin inhibitor (ITI). We have shown previously that cartilage LP is present in the maturing rat and mouse ovary. In the present study, we have employed immunohistochemistry to examine the anatomical distribution of cartilage LP i...
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Granulosa cell tumours are observed with increased frequency among calves slaughtered in Northern Italy. The use of illegal anabolics in breeding was taken into account as a cause of this pathology. An in vitro approach was used to detect the possible alterations of cell proliferation induced by anabolics on primary cultures of bovine granulosa-luteal cells. Cultures were treated with different...
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The luteal phase in the normal human menstrual cycle is known to be about 14 days. The physiological mechanisms that regulate the corpus luteum remain to be clarified, although apoptosis is reported to be involved. This study was undertaken to investigate the regulation of luteal function by gonadotropins, cytokines, and PGs, concentrating attention on the incidence of apoptosis and its molecul...
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ژورنال
عنوان ژورنال: The Journal of Clinical Endocrinology & Metabolism
سال: 1997
ISSN: 0021-972X,1945-7197
DOI: 10.1210/jcem.82.9.4240